NCBP2 antibody: A302-553A


Antibody Info
Product_IDA302-553A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB039KCI
Target Info
GeneNameNCBP2
Phosphate
Target_nameNCBP2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_GeneTex_GTX115648_40310_NCBP2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NCBP2 antibody (lanes under 'NCBP2').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NCBP2 antibody (lanes under 'NCBP2').

Method: immunoprecipitation

Releated Sample: eCLIP:477

Status: Released

Lab: Yeo Lab

NCBP2_Secondary_Western.png<br>Caption: Western blot following shRNA against NCBP2 in K562 and HepG2 whole cell lysate using NCBP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against NCBP2. Lanes 5-7 follow the same pattern, but in HepG2. NCBP2 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against NCBP2 in K562 and HepG2 whole cell lysate using NCBP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against NCBP2. Lanes 5-7 follow the same pattern, but in HepG2. NCBP2 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV08-35

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A302-553A_1_NCBP2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using NCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NCBP2 antibody (lanes under 'NCBP2').

Caption: IP-Western Blot analysis of K562 whole cell lysate using NCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NCBP2 antibody (lanes under 'NCBP2').

Method: immunoprecipitation

Releated Sample: eCLIP:566

Status: Released

Lab: Yeo Lab

NCBP2_Secondary_Western.png<br>Caption: Western blot following shRNA against NCBP2 in K562 and HepG2 whole cell lysate using NCBP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against NCBP2. Lanes 5-7 follow the same pattern, but in HepG2. NCBP2 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against NCBP2 in K562 and HepG2 whole cell lysate using NCBP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against NCBP2. Lanes 5-7 follow the same pattern, but in HepG2. NCBP2 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV08-35

Status: Released

Lab: Graveley Lab