Antibodies of DAZAP1


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DAZAP1Product_ID: A303-983A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DAZAP1-human		 HepG2_Bethyl_A303-983A_1_DAZAP1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DAZAP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DAZAP1 antibody (lanes under 'DAZAP1').
 DAZAP1_Secondary_Western.png<br>Caption: Western blot following shRNA against DAZAP1 in K562 and HepG2 whole cell lysate using DAZAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DAZAP1. Lanes 5-8 follow the same pattern, but in HepG2. DAZAP1 protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_Bethyl_A303-983A_1_DAZAP1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DAZAP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DAZAP1 antibody (lanes under 'DAZAP1').
K562_Bethyl_A303-983A_1_DAZAP1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DAZAP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DAZAP1 antibody (lanes under 'DAZAP1').
 DAZAP1_Secondary_Western.png<br>Caption: Western blot following shRNA against DAZAP1 in K562 and HepG2 whole cell lysate using DAZAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DAZAP1. Lanes 5-8 follow the same pattern, but in HepG2. DAZAP1 protein appears as the green band, Tubulin serves as a control and appears in red.
DAZAP1Product_ID: A303-984A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DAZAP1-human		 Bethyl_A303-984A_1_DAZAP1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DAZAP1 specific antibody, A303-984A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DAZAP1-specific antibody, A303-984A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DAZAP1-K562-A303-984A.png<br>Caption: Western blot following shRNA against DAZAP1 in K562 whole cell lysate using DAZAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DAZAP1. DAZAP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
DAZAP1Product_ID: A303-985A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DAZAP1-human		 Bethyl_A303-985A_1_DAZAP1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DAZAP1 specific antibody, A303-985A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DAZAP1-specific antibody, A303-985A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DAZAP1-K562-A303-985A.png<br>Caption: Western blot following shRNA against DAZAP1 in K562 whole cell lysate using DAZAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DAZAP1. DAZAP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.