GATA1      CRISPR in K562      Batch: BGKcLV53

Experiment Information (Status: Sequencing)
BGKcLV53-59BGKcLV53-60
idx00
TRCN#_or_BGC#BGC#0001508BGC#0001508
shRNA_or_gRNA_sequenceTGCGGCACTGGCCTACTACATGCGGCACTGGCCTACTACA
PAMGGGGGG
NameGATA1-90GATA1-90
Sample_IDBGKcLV53-59BGKcLV53-60
transduction_Date2/24/262/24/26
daysD6D6
RBP_nameGATA1GATA1
qPCR_result12.028.4
Ave_qPCR20.2
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc921650
WB_result88.891.3
Ave_WB
WB_DONE_date3/20/26,3/12/26
MW43kd
IP
antibody_Cat#710049RI243501
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID64216422




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
GATA1Product_ID: 710049
Lot_ID: RI243501
Source: Thermo Fisher
Target Name: GATA1-human		 ThermoFisher_710049_RI243501_GATA1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the GATA1 specific antibody, 710049. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the GATA1-specific antibody, 710049. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 GATA1-K562-CRISPR-710049.png<br>Caption: Western blot following CRISPR against GATA1 in K562 whole cell lysate using GATA1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GATA1. GATA1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGKcLV53-59BGKcLV53-60
Sample_IDBGKcLV53-59BGKcLV53-60
Sample Name
Sample NameGATA1-BGKcLV53-59GATA1-BGKcLV53-60
RBPGATA1GATA1
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2026-06-022026-06-02
ProjectENCORE2ENCORE2
Note
ID1479414795




Library-Prep Information
BGKcLV53-59BGKcLV53-60
Sample #4546
Sample NameGATA1-BGKcLV53-59GATA1-BGKcLV53-60
Sample_Name_Alias
Index Well PositionC07D07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-262026-05-26
Lib_IDLib-260526Lib-260526
Tecan_Location
Tecan
Tecan_date
Size_bp286290
Peak_Molarity63.5053.90
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-272026-05-27
Sample_IDBGKcLV53-59BGKcLV53-60
RBPGATA1GATA1
Batch_IDBGKcLV53BGKcLV53
WB_result88.80091.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48844885




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database