EDC4      CRISPR in K562      Batch: BGKcLV53

Experiment Information (Status: Sequencing)
BGKcLV53-43BGKcLV53-43BGKcLV53-44BGKcLV53-44
idx0000
TRCN#_or_BGC#BGC#0001461BGC#0001461BGC#0001461BGC#0001461
shRNA_or_gRNA_sequenceCTGCGCGAGCATCGACATCGCTGCGCGAGCATCGACATCGCTGCGCGAGCATCGACATCGCTGCGCGAGCATCGACATCG
PAMAGGAGGAGGAGG
NameEDC4_99BEDC4_99BEDC4_99BEDC4_99B
Sample_IDBGKcLV53-43BGKcLV53-43BGKcLV53-44BGKcLV53-44
transduction_Date2/24/262/24/262/24/262/24/26
daysD6D6D6D6
RBP_nameEDC4EDC4EDC4EDC4
qPCR_result45.555.9
Ave_qPCR50.7
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc3789378934793479
WB_result78.571.8
Ave_WB
WB_DONE_date3/17/265/5/26
MW152KD152KD
IP
antibody_Cat#A300-745AA300-746Alot# 2lot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID6403640564046406




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EDC4Product_ID: A300-745A
Lot_ID: 2
Source: Bethyl Labs
Target Name: EDC4-human		 EDC4-K562-CRISPR-A300-745A.png<br>Caption: Western blot following CRISPR against EDC4 in K562 whole cell lysate using EDC4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EDC4. EDC4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV53-43BGKcLV53-43BGKcLV53-44BGKcLV53-44
Sample_IDBGKcLV53-43BGKcLV53-43BGKcLV53-44BGKcLV53-44
Sample Name
Sample NameEDC4-BGKcLV53-43EDC4-BGKcLV53-44
RBPEDC4EDC4EDC4EDC4
Cell_LineK562K562K562K562
Exp UID
StatusSequencingNotSatisfiedSequencingNotSatisfied
Status_date2026-06-022026-05-182026-06-022026-05-18
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14792148441479314845




Library-Prep Information
BGKcLV53-43BGKcLV53-44
Sample #4344
Sample NameEDC4-BGKcLV53-43EDC4-BGKcLV53-44
Sample_Name_Alias
Index Well PositionA07B07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-262026-05-26
Lib_IDLib-260526Lib-260526
Tecan_Location
Tecan
Tecan_date
Size_bp292295
Peak_Molarity63.0070.50
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-272026-05-27
Sample_IDBGKcLV53-43BGKcLV53-44
RBPEDC4EDC4
Batch_IDBGKcLV53BGKcLV53
WB_result78.50071.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48824883




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database