ARPC1B      CRISPR in K562      Batch: BGKcLV53

Experiment Information (Status: LibPreping)
BGKcLV53-15BGKcLV53-15BGKcLV53-16BGKcLV53-16
idx0000
TRCN#_or_BGC#BGC#0001496BGC#0001496BGC#0001496BGC#0001496
shRNA_or_gRNA_sequenceATCCGCAGGATGACCAGCGTATCCGCAGGATGACCAGCGTATCCGCAGGATGACCAGCGTATCCGCAGGATGACCAGCGT
PAMGGGGGGGGGGGG
NameARPC1B_91ARPC1B_91ARPC1B_91ARPC1B_91
Sample_IDBGKcLV53-15BGKcLV53-15BGKcLV53-16BGKcLV53-16
transduction_Date2/24/262/24/262/24/262/24/26
daysD6D6D6D6
RBP_nameARPC1BARPC1BARPC1BARPC1B
qPCR_result61.063.6
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc4130413050385038
WB_result58.968.4
Ave_WB
WB_DONE_date3/10/265/5/26no clear band on the
MW42kDa42kDa
IP
antibody_Cat#A302-780AA302-781Alot# 3lot# 3
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID6373637563746376




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ARPC1BProduct_ID: A302-780A
Lot_ID: 3
Source: Bethyl Labs
Target Name: ARPC1B-human		 Bethyl_A302-780A_3_ARPC1B.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ARPC1B specific antibody, A302-780A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ARPC1B-specific antibody, A302-780A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ARPC1B-K562-CRISPR-A302-780A.png<br>Caption: Western blot following CRISPR against ARPC1B in K562 whole cell lysate using ARPC1B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ARPC1B. ARPC1B protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGKcLV53-15BGKcLV53-15BGKcLV53-16BGKcLV53-16
Sample_IDBGKcLV53-15BGKcLV53-15BGKcLV53-16BGKcLV53-16
Sample Name
Sample Name
RBPARPC1BARPC1BARPC1BARPC1B
Cell_LineK562K562K562K562
Exp UID
StatusLibPrepingNotSatisfiedLibPrepingNotSatisfied
Status_date2026-05-182026-05-182026-05-182026-05-18
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14788148181478914819




Library-Prep Information
BGKcLV53-15BGKcLV53-16
Sample #3940
Sample NameARPC1B-BGKcLV53-15ARPC1B-BGKcLV53-16
Sample_Name_Alias
Index Well PositionE06F06
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-262026-05-26
Lib_IDLib-260526Lib-260526
Tecan_Location
Tecan
Tecan_date
Size_bp
Peak_Molarity
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_method
SampleQC_date
Sample_IDBGKcLV53-15BGKcLV53-16
RBPARPC1BARPC1B
Batch_IDBGKcLV53BGKcLV53
WB_result58.90068.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusIndexAssignedIndexAssigned
ProjectENCORE2ENCORE2
ID48784879




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database