CANX      CRISPR in K562      Batch: BGKcLV51

Experiment Information (Status: LibPreping)
BGKcLV51-23BGKcLV51-23BGKcLV51-24BGKcLV51-24
idx0000
TRCN#_or_BGC#BGC#0000307BGC#0000307BGC#0000307BGC#0000307
shRNA_or_gRNA_sequenceCCTTGACGATGTCATTGAAGCCTTGACGATGTCATTGAAGCCTTGACGATGTCATTGAAGCCTTGACGATGTCATTGAAG
PAMaggaggaggagg
NameCANX_79CANX_79CANX_79CANX_79
Sample_IDBGKcLV51-23BGKcLV51-23BGKcLV51-24BGKcLV51-24
transduction_Date9/30/20259/30/20259/30/20259/30/2025
daysD6D6D6D6
RBP_nameCANXCANXCANXCANX
qPCR_result59.561.6
Ave_qPCR
RT-qPCR_primer-Fatgtggtggtgcctatgtga
RT-qPCR_primer-Rggggtcttgtcatggaactg
protein_conc2581258129362936
WB_result59.346.767.744.8472
Ave_WB57.7595
WB_DONE_date12/17/2512/17/25
MW68kd
IP
antibody_Cat#A303-694AA303-696Alot# 1lot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID6260626262616263




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CANXProduct_ID: A303-694A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CANX-human		 CANX-hepg2-CRISPR-A303-694A-LICOR.png<br>Caption: Western blot following CRISPR against CANX in HepG2 whole cell lysate using CANX specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CANX. CANX protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A303-694A_1_CANX.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CANX specific antibody, A303-694A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CANX-specific antibody, A303-694A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CANX-K562-CRISPR-A303-694A.png<br>Caption: Western blot following CRISPR against CANX in K562 whole cell lysate using CANX specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CANX. CANX protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV51-23BGKcLV51-23BGKcLV51-24BGKcLV51-24
Sample_IDBGKcLV51-23BGKcLV51-23BGKcLV51-24BGKcLV51-24
Sample Name
Sample Name
RBPCANXCANXCANXCANX
Cell_LineK562K562K562K562
Exp UID
StatusLibPrepingNotSatisfiedLibPrepingNotSatisfied
Status_date2026-05-132026-05-132026-05-132026-05-13
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14572146041457314605




Library-Prep Information
BGKcLV51-23BGKcLV51-24
Sample #78
Sample NameCANX-BGKcLV51-23CANX-BGKcLV51-24
Sample_Name_Alias
Index Well PositionG01H01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp286302
Peak_Molarity36.0038.60
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGKcLV51-23BGKcLV51-24
RBPCANXCANX
Batch_IDBGKcLV51BGKcLV51
WB_result59.30067.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48464847




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database