CALR      CRISPR in K562      Batch: BGKcLV51

Experiment Information (Status: LibPreping)
BGKcLV51-19BGKcLV51-20
idx00
TRCN#_or_BGC#BGC#0000170BGC#0000170
shRNA_or_gRNA_sequenceGTAGACGGCAGGCTCGGCGAGTAGACGGCAGGCTCGGCGA
PAMCGGCGG
NameCALR-91FCALR-91F
Sample_IDBGKcLV51-19BGKcLV51-20
transduction_Date9/30/20259/30/2025
daysD6D6
RBP_nameCALRCALR
qPCR_result75.475.9
Ave_qPCR
RT-qPCR_primer-Fccgttgcaaggatgatgagt
RT-qPCR_primer-Rcacctggctgttgtcaatctt
protein_conc26982840
WB_result52.355.4
Ave_WB
WB_DONE_date10/14/25
MW48kd
IP
antibody_Cat#A301-130Alot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID62566257




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CALRProduct_ID: A301-130A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CALR-human		 HepG2_Bethyl_A301-130A_1_CALR.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HepG2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-HepG2 antibody (lanes under 'HepG2').
 Calreticulin_Secondary_Western.png<br>Caption: Western blot following shRNA against Calreticulin in HepG2 whole cell lysate using Calreticulin specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against Calreticulin. Calreticulin protein appears as the green band, GAPDH serves as a control and appears in red.
 CALR-k562-CRISPR-A301-130A.png<br>Caption: Western blot following CRISPR against CALR in K562 whole cell lysate using CALR specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CALR. CALR protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV51-19BGKcLV51-20
Sample_IDBGKcLV51-19BGKcLV51-20
Sample Name
Sample Name
RBPCALRCALR
Cell_LineK562K562
Exp UID
StatusLibPrepingLibPreping
Status_date2026-05-132026-05-13
ProjectENCORE2ENCORE2
Note
ID1457014571




Library-Prep Information
BGKcLV51-19BGKcLV51-20
Sample #56
Sample NameCALR-BGKcLV51-19CALR-BGKcLV51-20
Sample_Name_Alias
Index Well PositionE01F01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp286284
Peak_Molarity53.5063.30
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGKcLV51-19BGKcLV51-20
RBPCALRCALR
Batch_IDBGKcLV51BGKcLV51
WB_result52.30055.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48444845




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database