SEPT7      CRISPR in K562      Batch: BGKcLV51

Experiment Information (Status: LibPreping)
BGKcLV51-105BGKcLV51-106
idx00
TRCN#_or_BGC#BGC#0001484BGC#0001484
shRNA_or_gRNA_sequenceAAGAACCTTGAAGGCTATGTAAGAACCTTGAAGGCTATGT
PAMGGGGGG
NameSEPTIN7_63SEPTIN7_63
Sample_IDBGKcLV51-105BGKcLV51-106
transduction_Date9/30/20259/30/2025
daysD6D6
RBP_nameSEPT7SEPT7
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-Faggatttggagatgcagtgg
RT-qPCR_primer-Raggcatctgacgtctgttca
protein_conc10071053
WB_result53.156.6
Ave_WB
WB_DONE_date11/19/25
MW
IP
antibody_Cat#A304-212Alot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID63466347




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SEPT7Product_ID: A304-212A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SEPT7-human		 SEPTIN7-HEPG2-CRISPR-A304-212A.png<br>Caption: Western blot following CRISPR against SEPT7 in HepG2 whole cell lysate using SEPT7 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SEPT7. SEPT7 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SEPTIN7-k562-CRISPR-A304-212A.png<br>Caption: Western blot following CRISPR against SEPT7 in K562 whole cell lysate using SEPT7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SEPT7. SEPT7 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV51-105BGKcLV51-106
Sample_IDBGKcLV51-105BGKcLV51-106
Sample Name
Sample Name
RBPSEPT7SEPT7
Cell_LineK562K562
Exp UID
StatusLibPrepingLibPreping
Status_date2026-05-132026-05-13
ProjectENCORE2ENCORE2
Note
ID1458414585




Library-Prep Information
BGKcLV51-105BGKcLV51-106
Sample #1920
Sample NameSEPT7-BGKcLV51-105SEPT7-BGKcLV51-106
Sample_Name_Alias
Index Well PositionC03D03
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp291288
Peak_Molarity47.1054.60
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGKcLV51-105BGKcLV51-106
RBPSEPT7SEPT7
Batch_IDBGKcLV51BGKcLV51
WB_result53.10056.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48584859




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database