NUFIP2      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequencing)
BGKcLV48-41BGKcLV48-42
idx00
TRCN#_or_BGC#BGC#0001036BGC#0001036
shRNA_or_gRNA_sequenceGTCCCAGCCGCTTTCAATGGGTCCCAGCCGCTTTCAATGG
PAMAGGAGG
NameNUFIP2_88NUFIP2_88
Sample_IDBGKcLV48-41BGKcLV48-42
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameNUFIP2NUFIP2
qPCR_result33.617.7
Ave_qPCR25.7
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc15461624
WB_result66.064.8
Ave_WB
WB_DONE_date5/1/25
MW76kd
IP
antibody_Cat#A301-600Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61706171




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NUFIP2Product_ID: A301-600A
Lot_ID: 1
Source: Bethyl Labs
Target Name: NUFIP2-human		 NUFIP2-K562-CRISPR-A301-600A.png<br>Caption: Western blot following CRISPR against NUFIP2 in K562 whole cell lysate using NUFIP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NUFIP2. NUFIP2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-41BGKcLV48-42
Sample_IDBGKcLV48-41BGKcLV48-42
Sample Name
Sample NameNUFIP2-BGKcLV48-41NUFIP2-BGKcLV48-42
RBPNUFIP2NUFIP2
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2025-09-102025-09-10
ProjectENCORE2ENCORE2
Note
ID1424414245




Library-Prep Information
BGKcLV48-41BGKcLV48-42
Sample #78
Sample NameNUFIP2-BGKcLV48-41NUFIP2-BGKcLV48-42
Sample_Name_Alias
Index Well PositionG01H01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp254254
Peak_Molarity50.5041.40
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-41BGKcLV48-42
RBPNUFIP2NUFIP2
Batch_IDBGKcLV48BGKcLV48
WB_result66.00064.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47524753




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database