RPL7      CRISPR in K562      Control: NT-BGKcLV46-1,NT-BGKcLV46-2

General Information
RBPRPL7
Cell_LineK562
MethodCRISPR
Exp_NameRPL7-BGKcLV46-K562
ENCODE_series_ID
Batch_IDBGKcLV46
Pool IDPool-250312
Local_Set_Nameset77
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1RPL7-BGKcLV46-65
Rep2RPL7-BGKcLV46-66
CN1NT-BGKcLV46-1
CN2NT-BGKcLV46-2
Rep1_qPCR68.3
Rep2_qPCR64.0
Rep1_WB52.6
Rep2_WB54.6065
Antibody Cat#A300-741A
Antibody Lot#lot# 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1649




Experiment Information (Status: Submitting)
BGKcLV46-65
idx0
TRCN#_or_BGC#BGC#0001259
shRNA_or_gRNA_sequenceTTCAGCTTCGAAAGGCAAGG
PAMAGG
NameRPL7-61
Sample_IDBGKcLV46-65
transduction_Date12/20/24
daysD6
RBP_nameRPL7
qPCR_result68.3
Ave_qPCR66.2
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc3510
WB_result52.6
Ave_WB
WB_DONE_date1/2/24,1/6/25,1/17/2
MW29kd
IP
antibody_Cat#A300-741A
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0
Rep2_TPM0
ActionReady
Library_start_date
repeat_library
Note
ID6061




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPL7Product_ID: A300-741A
Lot_ID: 3
Source: Bethyl Labs
Target Name: RPL7-human
RPL7-K562-CRISPR-A300-741A.png<br>Caption: Western blot following CRISPR against RPL7 in K562 whole cell lysate using RPL7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPL7. RPL7 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV46-65BGKcLV46-66
Sample_IDBGKcLV46-65BGKcLV46-66
Sample Name
Sample NameRPL7-BGKcLV46-65RPL7-BGKcLV46-66
RBPRPL7RPL7
Cell_LineK562K562
Exp UID
StatusSubmittingSubmitting
Status_date2025-04-212025-04-21
ProjectENCORE2ENCORE2
Note
ID1405214053




Library-Prep Information
BGKcLV46-65BGKcLV46-66
Sample #3132
Sample NameRPL7-BGKcLV46-65RPL7-BGKcLV46-66
Sample_Name_Alias
Index Well PositionG04H04
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-02-202025-02-20
Lib_IDLib-250220Lib-250220
Tecan_Location
Tecan
Tecan_date
Size_bp298302
Peak_Molarity73.0063.30
libSampleQC_DNA_WellDNA_Library_set77/set1/G10.pngDNA_Library_set77/set1/H10.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set77/set1/G4.pngRNA_Library_set77/set1/H4.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-02-282025-02-28
Sample_IDBGKcLV46-65BGKcLV46-66
RBPRPL7RPL7
Batch_IDBGKcLV46BGKcLV46
WB_result52.60054.607
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46904691




Sequencing Information
BGKcLV46-65BGKcLV46-66
Sample_IDBGKcLV46-65BGKcLV46-66
Sample NameRPL7-BGKcLV46-65RPL7-BGKcLV46-66
Pool IDPool-250312Pool-250312
LocalServer_folderset77set77
total_reads55,023,41868,889,835
total_aligned_reads51,257,18764,602,312
unique_aligned_reads47,254,06959,646,025
percent_uniqueAligned0.858800.86582
correlation_replicates0.9976920.997692
spikein_reads57,004132,214
percent_spikeins0.001040.00192
original_ReadLength101101
QC_StatusSubmittingSubmitting
ID33953396




Data Submission Information
DCC submission information was not avaliable




File Information
file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
RPL7-BGKcLV46-65_S31_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set77/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3395NovaSeq6000100paired-ended1NT-BGKcLV46-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV46-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gzENCORE232180
RPL7-BGKcLV46-65_S31_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set77/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3395NovaSeq6000100paired-ended2NT-BGKcLV46-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV46-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE232230
RPL7-BGKcLV46-65_Aligned.sortedByCoord.out.bamENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3395GRCh38V40RPL7-BGKcLV46-65_S31_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPL7-BGKcLV46-65_S31_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE232280
RPL7-BGKcLV46-65_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3395GRCh38V40RPL7-BGKcLV46-65_Aligned.sortedByCoord.out.bamENCORE232330
RPL7-BGKcLV46-65_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3395GRCh38V40RPL7-BGKcLV46-65_Aligned.sortedByCoord.out.bamENCORE232380
RPL7-BGKcLV46-65_Signal.Unique.strand-.bwENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3395GRCh38V40RPL7-BGKcLV46-65_Aligned.sortedByCoord.out.bamENCORE232430
RPL7-BGKcLV46-65_Signal.Unique.strand+.bwENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3395GRCh38V40RPL7-BGKcLV46-65_Aligned.sortedByCoord.out.bamENCORE232480
RPL7-BGKcLV46-65_quant.sfENCODE_DATA/set77/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3395GRCh38V40RPL7-BGKcLV46-65_S31_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPL7-BGKcLV46-65_S31_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE232530
RPL7-BGKcLV46-66_S32_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set77/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3396NovaSeq6000100paired-ended1NT-BGKcLV46-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV46-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gzENCORE232181
RPL7-BGKcLV46-66_S32_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set77/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3396NovaSeq6000100paired-ended2NT-BGKcLV46-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV46-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE232231
RPL7-BGKcLV46-66_Aligned.sortedByCoord.out.bamENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3396GRCh38V40RPL7-BGKcLV46-66_S32_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPL7-BGKcLV46-66_S32_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE232281
RPL7-BGKcLV46-66_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3396GRCh38V40RPL7-BGKcLV46-66_Aligned.sortedByCoord.out.bamENCORE232331
RPL7-BGKcLV46-66_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3396GRCh38V40RPL7-BGKcLV46-66_Aligned.sortedByCoord.out.bamENCORE232381
RPL7-BGKcLV46-66_Signal.Unique.strand-.bwENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3396GRCh38V40RPL7-BGKcLV46-66_Aligned.sortedByCoord.out.bamENCORE232431
RPL7-BGKcLV46-66_Signal.Unique.strand+.bwENCODE_DATA/set77/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3396GRCh38V40RPL7-BGKcLV46-66_Aligned.sortedByCoord.out.bamENCORE232481
RPL7-BGKcLV46-66_quant.sfENCODE_DATA/set77/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3396GRCh38V40RPL7-BGKcLV46-66_S32_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPL7-BGKcLV46-66_S32_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE232531