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SMYD3
CRISPR
in K562
Control:
NT-BGKcLV19
General Information
Experiment Information
Sequencing Information
Data Submission
Files
General Information
RBP
SMYD3
Cell_Line
K562
Method
CRISPR
Exp_Name
SMYD3-BGKcLV19-K562
ENCODE_series_ID
ENCSR441UTQ
Batch_ID
BGKcLV19
Pool ID
Pool-200928
Local_Set_Name
set45_K
ENCODE_KD_Exp_ID
ENCSR775USN
ENCODE_CN_Exp_ID
ENCSR978MMZ
Rep1
SMYD3-BGKcLV19-51
Rep2
SMYD3-BGKcLV19-52
CN1
NT-BGKcLV19-1
CN2
NT-BGKcLV19-2
Rep1_qPCR
31.23
Rep2_qPCR
45.08
Rep1_WB
91.4
Rep2_WB
90.6
Antibody Cat#
PA5-31919
Antibody Lot#
Lot# UA2701668D
Antibody DCC ID
ENCAB101UYP
Status
Released
Project
ENCODE4
ID
60
Experiment Information
Western Blot
Experiment Status
Experiment Information (Status:
NotSatisfied
)
BGKcLV19-49
BGKcLV19-50
idx
723
724
TRCN#_or_BGC#
BGC#0000429
BGC#0000429
shRNA_or_gRNA_sequence
GCAGTCCGAGACATCGAGGT
GCAGTCCGAGACATCGAGGT
PAM
GGG
GGG
Name
SMYD3_89
SMYD3_89
Sample_ID
BGKcLV19-49
BGKcLV19-50
transduction_Date
11/6/19
11/6/19
days
Day 6
Day 6
RBP_name
SMYD3
SMYD3
qPCR_result
86.29
82.47
Ave_qPCR
RT-qPCR_primer-F
AGACATCGAGGTGGGAGAGG
RT-qPCR_primer-R
GGCAACGGAAACAGTCACAT
protein_conc
3387
3541
WB_result
88.7
89.2
Ave_WB
89.0
WB_DONE_date
12/5/19
MW
49kd
IP
Rabbit / I
antibody_Cat#
PA5-31919
Antibody DCC ID
no ID
submitted_to_DCC_date
Rep1_TPM
101.8
0
Rep2_TPM
103.9
0
Action
Library_start_date
repeat_library
Note
ID
4843
4844
Western Blot
RBP
Antibody Info
Primary-HepG2
Secondary-HepG2
Primary-K562
Secondary-K562
Primary-UBERON
Primary-Hela
SMYD3
Product_ID:
PA5-31919
Lot_ID: UA2701668D
Source: Thermo Fisher
Target Name: SMYD3-human
Experiment Status
BGKcLV19-49
BGKcLV19-50
Sample_ID
BGKcLV19-49
BGKcLV19-50
Sample Name
Sample Name
RBP
SMYD3
SMYD3
Cell_Line
K562
K562
Exp UID
Status
NotSatisfied
NotSatisfied
Status_date
2020-09-17
2020-09-17
Project
ENCODE4
ENCODE4
Note
ID
5749
5750
Library-Prep
Sequencing
Library-Prep Information
Library info was not avaliable
Sequencing Information
Sequencing info was not avaliable
Data Submission Information
DCC submission information was not avaliable
File Information
Files of the experiment was not avaliable in the database
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