CANX      CRISPR in HepG2      Batch: BGHcLV52

Experiment Information (Status: LibPreping)
BGHcLV52-9BGHcLV52-9BGHcLV52-9BGHcLV52-10BGHcLV52-10BGHcLV52-10
idx000000
TRCN#_or_BGC#BGC#0000462BGC#0000462BGC#0000462BGC#0000462BGC#0000462BGC#0000462
shRNA_or_gRNA_sequenceTCGTATAAGGGGTCTTGTCATCGTATAAGGGGTCTTGTCATCGTATAAGGGGTCTTGTCATCGTATAAGGGGTCTTGTCATCGTATAAGGGGTCTTGTCATCGTATAAGGGGTCTTGTCA
PAMTGGTGGTGGTGGTGGTGG
NameCANX_89CANX_89CANX_89CANX_89CANX_89CANX_89
Sample_IDBGHcLV52-9BGHcLV52-9BGHcLV52-9BGHcLV52-10BGHcLV52-10BGHcLV52-10
transduction_Date1/8/261/8/261/8/261/8/261/8/261/8/26
daysD6D6D6D6D6D6
RBP_nameCANXCANXCANXCANXCANXCANX
qPCR_result78.3387678.3387679.3210579.32105
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc284528452845269726972697
WB_result52.775.756.273.4
Ave_WB
WB_DONE_date1/23/261/30/262/17/26no band
MW68kd68kd
IP
antibody_Cat#A303-694AA303-696AMA3-027lot# 1lot# 1lot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM000000
Rep2_TPM000000
ActionReadyReady
Library_start_date
repeat_library
Note
ID101381014010142101391014110143




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CANXProduct_ID: A303-694A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CANX-human		 CANX-hepg2-CRISPR-A303-694A-LICOR.png<br>Caption: Western blot following CRISPR against CANX in HepG2 whole cell lysate using CANX specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CANX. CANX protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A303-694A_1_CANX.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CANX specific antibody, A303-694A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CANX-specific antibody, A303-694A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CANX-K562-CRISPR-A303-694A.png<br>Caption: Western blot following CRISPR against CANX in K562 whole cell lysate using CANX specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CANX. CANX protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
CANXProduct_ID: A303-696A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CANX-human		 CANX-hepg2-CRISPR-A303-696A-LICOR.png<br>Caption: Western blot following CRISPR against CANX in HepG2 whole cell lysate using CANX specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CANX. CANX protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A303-696A_1_CANX.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CANX specific antibody, A303-696A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CANX-specific antibody, A303-696A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.




Experiment Status
BGHcLV52-9BGHcLV52-9BGHcLV52-9BGHcLV52-10BGHcLV52-10BGHcLV52-10
Sample_IDBGHcLV52-9BGHcLV52-9BGHcLV52-9BGHcLV52-10BGHcLV52-10BGHcLV52-10
Sample Name
Sample Name
RBPCANXCANXCANXCANXCANXCANX
Cell_LineHepG2HepG2HepG2HepG2HepG2HepG2
Exp UID
StatusLibPrepingNotSatisfiedNotSatisfiedLibPrepingNotSatisfiedNotSatisfied
Status_date2026-05-132026-05-132026-05-132026-05-132026-05-132026-05-13
ProjectENCORE2ENCORE2ENCORE2ENCORE2ENCORE2ENCORE2
Note
ID146901471014712146911471114713




Library-Prep Information
BGHcLV52-9BGHcLV52-10
Sample #2324
Sample NameCANX-BGHcLV52-9CANX-BGHcLV52-10
Sample_Name_Alias
Index Well PositionG03H03
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp281282
Peak_Molarity38.2037.40
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGHcLV52-9BGHcLV52-10
RBPCANXCANX
Batch_IDBGHcLV52BGHcLV52
WB_result75.70073.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48624863




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database