RPS6      CRISPR in HepG2      Batch: BGHcLV52

Experiment Information (Status: LibPreping)
BGHcLV52-59BGHcLV52-60
idx00
TRCN#_or_BGC#BGC#0001303BGC#0001303
shRNA_or_gRNA_sequenceCTGACGCTCTGGGTGAAGAACTGACGCTCTGGGTGAAGAA
PAMTGGTGG
NameRPS6_64RPS6_64
Sample_IDBGHcLV52-59BGHcLV52-60
transduction_Date1/8/261/8/26
daysD6D6
RBP_nameRPS6RPS6
qPCR_result66.7549271.99086
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc23841799
WB_result61.463.1
Ave_WB
WB_DONE_date2/5/26
MW
IP29kDfu's
antibody_Cat#A300-556Alot# 2
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID1019610197




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPS6Product_ID: A300-556A
Lot_ID: 2
Source: Bethyl Labs
Target Name: RPS6-human		 RPS6-HEPG2-CRISPR-A300-556A-LICOR.png<br>Caption: Western blot following CRISPR against RPS6 in HepG2 whole cell lysate using RPS6 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6. RPS6 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A300-556A_2_RPS6.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RPS6 specific antibody, A300-556A. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the RPS6-specific antibody, A300-556A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RPS6-K562-CRISPR.png<br>Caption: Western blot following CRISPR against RPS6 in K562 whole cell lysate using RPS6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6. RPS6 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
RPS6-K562-CRISPR-A300-556A.png<br>Caption: Western blot following CRISPR against RPS6 in K562 whole cell lysate using RPS6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6. RPS6 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGHcLV52-59BGHcLV52-60
Sample_IDBGHcLV52-59BGHcLV52-60
Sample Name
Sample Name
RBPRPS6RPS6
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2026-05-132026-05-13
ProjectENCORE2ENCORE2
Note
ID1469614697




Library-Prep Information
BGHcLV52-59BGHcLV52-60
Sample #2930
Sample NameRPS6-BGHcLV52-59RPS6-BGHcLV52-60
Sample_Name_Alias
Index Well PositionE04F04
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp306290
Peak_Molarity60.8057.20
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGHcLV52-59BGHcLV52-60
RBPRPS6RPS6
Batch_IDBGHcLV52BGHcLV52
WB_result61.40063.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48684869




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database