SMG8      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-99BGHcLV49-100
idx00
TRCN#_or_BGC#BGC#0000873BGC#0000873
shRNA_or_gRNA_sequenceCGCGGAAGGTAACCGAACTGCGCGGAAGGTAACCGAACTG
PAMAGGAGG
NameSMG8_97SMG8_97
Sample_IDBGHcLV49-99BGHcLV49-100
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameSMG8SMG8
qPCR_result21.911.6
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc51074302
WB_result59.377.1
Ave_WB
WB_DONE_date7/10/25
MW110kd
IP
antibody_Cat#A301-471ALOT #1
Antibody DCC IDENCAB236BQW
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99859986




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SMG8Product_ID: A301-471A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SMG8-human		 SMG8-hepg2-CRISPR-A301-471A-LICOR.png<br>Caption: Western blot following CRISPR against SMG8 in HepG2 whole cell lysate using SMG8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG8. SMG8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SMG8-K562-CRISPR-A301-471A.png<br>Caption: Western blot following CRISPR against SMG8 in K562 whole cell lysate using SMG8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG8. SMG8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-99BGHcLV49-100
Sample_IDBGHcLV49-99BGHcLV49-100
Sample Name
Sample NameSMG8-BGHcLV49-99SMG8-BGHcLV49-100
RBPSMG8SMG8
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1436414365




Library-Prep Information
BGHcLV49-99BGHcLV49-100
Sample #6162
Sample NameSMG8-BGHcLV49-99SMG8-BGHcLV49-100
Sample_Name_Alias
Index Well PositionG08H08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp264251
Peak_Molarity68.0052.50
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-99BGHcLV49-100
RBPSMG8SMG8
Batch_IDBGHcLV49BGHcLV49
WB_result59.30077.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48064807




Sequencing Information
BGHcLV49-99BGHcLV49-100
Sample_IDBGHcLV49-99BGHcLV49-100
Sample NameSMG8-BGHcLV49-99SMG8-BGHcLV49-100
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35313532




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database