FTSJ3      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequencing)
BGHcLV49-67BGHcLV49-67BGHcLV49-68BGHcLV49-68
idx0000
TRCN#_or_BGC#BGC#0001027BGC#0001027BGC#0001027BGC#0001027
shRNA_or_gRNA_sequenceAGTGTTACGAACCATCCCCCAGTGTTACGAACCATCCCCCAGTGTTACGAACCATCCCCCAGTGTTACGAACCATCCCCC
PAMTGGTGGTGGTGG
NameFTSJ3_93FTSJ3_93FTSJ3_93FTSJ3_93
Sample_IDBGHcLV49-67BGHcLV49-67BGHcLV49-68BGHcLV49-68
transduction_Date6/6/256/6/256/6/256/6/25
daysD6D6D6D6
RBP_nameFTSJ3FTSJ3FTSJ3FTSJ3
qPCR_result39.244.2
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc3345334533593359
WB_result82.881.3
Ave_WB
WB_DONE_date7/3/257/21/25no band on the posio
MW97kd97kd
IP
antibody_Cat#A304-199AA304-198Alot # 1lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID9949995199509952




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
FTSJ3Product_ID: A304-199A
Lot_ID: 1
Source: Bethyl Labs
Target Name: FTSJ3-human		 FTSJ3-hepg2-CRISPR-A304-199A-LICOR.png<br>Caption: Western blot following CRISPR against FTSJ3 in HepG2 whole cell lysate using FTSJ3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FTSJ3. FTSJ3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 FTSJ3-K562-CRISPR-A304-199A.png<br>Caption: Western blot following CRISPR against FTSJ3 in K562 whole cell lysate using FTSJ3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FTSJ3. FTSJ3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-67BGHcLV49-67BGHcLV49-68BGHcLV49-68
Sample_IDBGHcLV49-67BGHcLV49-67BGHcLV49-68BGHcLV49-68
Sample Name
Sample NameFTSJ3-BGHcLV49-67FTSJ3-BGHcLV49-68
RBPFTSJ3FTSJ3FTSJ3FTSJ3
Cell_LineHepG2HepG2HepG2HepG2
Exp UID
StatusSequencingNotSatisfiedSequencingNotSatisfied
Status_date2025-09-292025-08-122025-09-292025-08-12
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14354144101435514411




Library-Prep Information
BGHcLV49-67BGHcLV49-68
Sample #5152
Sample NameFTSJ3-BGHcLV49-67FTSJ3-BGHcLV49-68
Sample_Name_Alias
Index Well PositionE07F07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp262255
Peak_Molarity81.8099.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-67BGHcLV49-68
RBPFTSJ3FTSJ3
Batch_IDBGHcLV49BGHcLV49
WB_result82.80081.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47964797




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database