EFL1      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-49BGHcLV49-50
idx00
TRCN#_or_BGC#BGC#0000953BGC#0000953
shRNA_or_gRNA_sequenceTTAACAATAGACTTCAGCTATTAACAATAGACTTCAGCTA
PAMTGGTGG
NameEFTUD1_71EFTUD1_71
Sample_IDBGHcLV49-49BGHcLV49-50
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameEFL1EFL1
qPCR_result63.663.2
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc48993845
WB_result82.692.9
Ave_WB
WB_DONE_date6/23/25
MW125KD
IP
antibody_Cat#A305-854A-Mlot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99319932




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EFL1Product_ID: A305-854A-M
Lot_ID: 1
Source: Bethyl Labs
Target Name: EFL1-human		 EFTUD1-hepg2-CRISPR-A305-854A-M-LICOR.png<br>Caption: Western blot following CRISPR against EFL1 in HepG2 whole cell lysate using EFL1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EFL1. EFL1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EFL1-K562-CRISPR-A305-854A-M.png<br>Caption: Western blot following CRISPR against EFL1 in K562 whole cell lysate using EFL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EFL1. EFL1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-49BGHcLV49-50
Sample_IDBGHcLV49-49BGHcLV49-50
Sample Name
Sample NameEFL1-BGHcLV49-49EFL1-BGHcLV49-50
RBPEFL1EFL1
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-10-302025-10-30
ProjectENCORE2ENCORE2
Note
ID1434614347




Library-Prep Information
BGHcLV49-49BGHcLV49-50
Sample #4344
Sample NameEFL1-BGHcLV49-49EFL1-BGHcLV49-50
Sample_Name_Alias
Index Well PositionE06F06
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp277266
Peak_Molarity90.0075.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-49BGHcLV49-50
RBPEFL1EFL1
Batch_IDBGHcLV49BGHcLV49
WB_result82.60092.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47884789




Sequencing Information
BGHcLV49-49BGHcLV49-50
Sample_IDBGHcLV49-49BGHcLV49-50
Sample NameEFL1-BGHcLV49-49EFL1-BGHcLV49-50
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35153516




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database