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FEN1
CRISPR
in HepG2
Control:
NT-BGHcLV41-1,NT-BGHcLV41-2
General Information
Experiment Information
Sequencing Information
Data Submission
Files
General Information
RBP
FEN1
Cell_Line
HepG2
Method
CRISPR
Exp_Name
FEN1-BGHcLV41-HepG2
ENCODE_series_ID
Batch_ID
BGHcLV41
Pool ID
Pool-240314
Local_Set_Name
set70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1
FEN1-BGHcLV41-71
Rep2
FEN1-BGHcLV41-72
CN1
NT-BGHcLV41-1
CN2
NT-BGHcLV41-2
Rep1_qPCR
53.0
Rep2_qPCR
57.0
Rep1_WB
76.2
Rep2_WB
81.3
Antibody Cat#
A300-255A
Antibody Lot#
lot # 3
Antibody DCC ID
Status
Submitted
Project
ENCORE2
ID
1459
Experiment Information
Western Blot
Experiment Status
Experiment Information (Status:
Submitting
)
BGHcLV41-71
BGHcLV41-72
idx
1344
1345
TRCN#_or_BGC#
BGC#0001017
BGC#0001017
shRNA_or_gRNA_sequence
GACTACTGTGAGAGTATCCG
GACTACTGTGAGAGTATCCG
PAM
GGG
GGG
Name
FEN1_93
FEN1_93
Sample_ID
BGHcLV41-71
BGHcLV41-72
transduction_Date
1/12/24
1/12/24
days
D6
D6
RBP_name
FEN1
FEN1
qPCR_result
53.0
57.0
Ave_qPCR
55.0
RT-qPCR_primer-F
ACTAAGCGGCTGGTGAAGGTCA
RT-qPCR_primer-R
GCAGCATAGACTTTGCCAGCCT
protein_conc
2294
1666
WB_result
76.2
81.3
Ave_WB
78.8
WB_DONE_date
1/24/23
MW
43kd
IP
antibody_Cat#
A300-255A
lot # 3
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM
0
0
Rep2_TPM
0
0
Action
Ready
Ready
Library_start_date
repeat_library
Note
ID
9587
9588
Western Blot
RBP
Antibody Info
Primary-HepG2
Secondary-HepG2
Primary-K562
Secondary-K562
Primary-UBERON
Primary-Hela
FEN1
Product_ID:
A300-255A
Lot_ID: 3
Source: Bethyl Labs
Target Name: FEN1-human
Experiment Status
BGHcLV41-71
BGHcLV41-72
Sample_ID
BGHcLV41-71
BGHcLV41-72
Sample Name
Sample Name
FEN1-BGHcLV41-71
FEN1-BGHcLV41-72
RBP
FEN1
FEN1
Cell_Line
HepG2
HepG2
Exp UID
Status
Submitting
Submitting
Status_date
2024-09-20
2024-09-20
Project
ENCORE2
ENCORE2
Note
ID
12978
12979
Library-Prep
Sequencing
Library-Prep Information
BGHcLV41-71
BGHcLV41-72
Sample #
31
32
Sample Name
FEN1-BGHcLV41-71
FEN1-BGHcLV41-72
Sample_Name_Alias
Index Well Position
G04
H04
Index_table
IDT_UniqueDualIndex_96
IDT_UniqueDualIndex_96
LibPrep_date
2024-03-01
2024-03-01
Lib_ID
Lib-240301
Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp
289
291
Peak_Molarity
77.80
64.20
libSampleQC_DNA_Well
DNA_Library_set70/set1/G4.png
DNA_Library_set70/set1/H4.png
RIN
9.9
10.0
libSample_RNA_Well
RNA_Library_set70/set1/C10.png
RNA_Library_set70/set1/D10.png
SampleQC_method
TapeStation_2022
TapeStation_2022
SampleQC_date
2024-03-14
2024-03-14
Sample_ID
BGHcLV41-71
BGHcLV41-72
RBP
FEN1
FEN1
Batch_ID
BGHcLV41
BGHcLV41
WB_result
76.200
81.300
Library Description
TruSeq mRNA
TruSeq mRNA
Repeat_Library_Suffix
Lib_Status
SendToSequence
SendToSequence
Project
ENCORE2
ENCORE2
ID
4256
4257
Sequencing Information
BGHcLV41-71
BGHcLV41-72
Sample_ID
BGHcLV41-71
BGHcLV41-72
Sample Name
FEN1-BGHcLV41-71
FEN1-BGHcLV41-72
Pool ID
Pool-240314
Pool-240314
LocalServer_folder
set70
set70
total_reads
41,051,233
52,317,218
total_aligned_reads
37,955,623
49,439,364
unique_aligned_reads
35,906,020
46,750,360
percent_uniqueAligned
0.87466
0.89359
correlation_replicates
0.998492
0.998492
spikein_reads
4,542
11,468
percent_spikeins
0.00011
0.00022
original_ReadLength
151
151
QC_Status
Submitting
Submitting
ID
2975
2976
Data Submission Information
DCC submission information was not avaliable
File Information
Files of the experiment was not avaliable in the database
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