EML4      CRISPR in HepG2      Control: NT-BGHcLV41-1,NT-BGHcLV41-2

General Information
RBPEML4
Cell_LineHepG2
MethodCRISPR
Exp_NameEML4-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EML4-BGHcLV41-41
Rep2EML4-BGHcLV41-42
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR38.8
Rep2_qPCR43.1
Rep1_WB91.0
Rep2_WB92.1
Antibody Cat#A301-909A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1454




Experiment Information (Status: Submitting)
BGHcLV41-41BGHcLV41-41BGHcLV41-42BGHcLV41-42
idx1310131213111313
TRCN#_or_BGC#BGC#0000987BGC#0000987BGC#0000987BGC#0000987
shRNA_or_gRNA_sequenceGCCGTACTCACCGAGACTGCGCCGTACTCACCGAGACTGCGCCGTACTCACCGAGACTGCGCCGTACTCACCGAGACTGC
PAMCGGCGGCGGCGG
NameEML4_93EML4_93EML4_93EML4_93
Sample_IDBGHcLV41-41BGHcLV41-41BGHcLV41-42BGHcLV41-42
transduction_Date1/12/241/12/241/12/241/12/24
daysD6D6D6D6
RBP_nameEML4EML4EML4EML4
qPCR_result38.838.843.143.1
Ave_qPCR41.041.0
RT-qPCR_primer-Ftgacatcagaacggaactgctgacatcagaacggaactgc
RT-qPCR_primer-Rcggtcggaagaaggtaaacacggtcggaagaaggtaaaca
protein_conc3898389835053505
WB_result91.0MULTIPLE BANDS92.1
Ave_WB91.6
WB_DONE_date2/1/242/29/24
MW109kd109kd
IP
antibody_Cat#A301-909AA301-908Alot # 1lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID9553955595549556




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EML4Product_ID: A301-909A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EML4-human		 EML4-HEPG2-CRISPR-A301-909A.png<br>Caption: Western blot following CRISPR against EML4 in HepG2 whole cell lysate using EML4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EML4. EML4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EML4-K562-CRISPR-A301-909A.png<br>Caption: Western blot following CRISPR against EML4 in K562 whole cell lysate using EML4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EML4. EML4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-41BGHcLV41-42
Sample_IDBGHcLV41-41BGHcLV41-42
Sample Name
Sample NameEML4-BGHcLV41-41EML4-BGHcLV41-42
RBPEML4EML4
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1296812969




Library-Prep Information
BGHcLV41-41BGHcLV41-42
Sample #2122
Sample NameEML4-BGHcLV41-41EML4-BGHcLV41-42
Sample_Name_Alias
Index Well PositionE03F03
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp299296
Peak_Molarity98.5094.00
libSampleQC_DNA_WellDNA_Library_set70/set1/E3.pngDNA_Library_set70/set1/F3.png
RIN9.99.9
libSample_RNA_WellRNA_Library_set70/set1/A9.pngRNA_Library_set70/set1/B9.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-41BGHcLV41-42
RBPEML4EML4
Batch_IDBGHcLV41BGHcLV41
WB_result91.00092.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42464247




Sequencing Information
BGHcLV41-41BGHcLV41-42
Sample_IDBGHcLV41-41BGHcLV41-42
Sample NameEML4-BGHcLV41-41EML4-BGHcLV41-42
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads51,136,20138,943,167
total_aligned_reads47,534,47636,761,168
unique_aligned_reads44,922,33334,775,195
percent_uniqueAligned0.878480.89297
correlation_replicates0.9990460.999046
spikein_reads6,0824,994
percent_spikeins0.000120.00013
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29652966




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database