EIF3K      CRISPR in HepG2      Control: NT-BGHcLV41-1,NT-BGHcLV41-2

General Information
RBPEIF3K
Cell_LineHepG2
MethodCRISPR
Exp_NameEIF3K-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF3K-BGHcLV41-33
Rep2EIF3K-BGHcLV41-34
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR85.6
Rep2_qPCR80.0
Rep1_WB68.1
Rep2_WB80.0
Antibody Cat#A301-763A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1452




Experiment Information (Status: Submitting)
BGHcLV41-33BGHcLV41-33BGHcLV41-34BGHcLV41-34
idx1302130213031303
TRCN#_or_BGC#BGC#0000972BGC#0000972BGC#0000972BGC#0000972
shRNA_or_gRNA_sequenceGTGCAAGTGCATGATCGACCGTGCAAGTGCATGATCGACCGTGCAAGTGCATGATCGACCGTGCAAGTGCATGATCGACC
PAMAGGAGGAGGAGG
NameEIF3K_96EIF3K_96EIF3K_96EIF3K_96
Sample_IDBGHcLV41-33BGHcLV41-33BGHcLV41-34BGHcLV41-34
transduction_Date1/12/241/12/241/12/241/12/24
daysD6D6D6D6
RBP_nameEIF3KEIF3KEIF3KEIF3K
qPCR_result85.685.680.080.0
Ave_qPCR82.882.8
RT-qPCR_primer-Fccagccttctttcagaccacccagccttctttcagaccac
RT-qPCR_primer-Rtccccgaggtacaaaatctgtccccgaggtacaaaatctg
protein_conc3793379331963196
WB_result68.156.180.050.5
Ave_WB74.053.3
WB_DONE_date1/24/246/28/24
MW25kd25kd
IP
antibody_Cat#A301-763AA301-762Alot # 1lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID9543954595449546




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF3KProduct_ID: A301-763A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EIF3K-human		 EIF3K-HEPG2-CRISPR-A301-763A.png<br>Caption: Western blot following CRISPR against EIF3K in HepG2 whole cell lysate using EIF3K specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF3K. EIF3K protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EIF3K-K562-CRISPR-A301-763A.png<br>Caption: Western blot following CRISPR against EIF3K in K562 whole cell lysate using EIF3K specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF3K. EIF3K protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
IARSProduct_ID: A304-747A
Lot_ID: 1
Source: Bethyl Labs
Target Name: IARS-human		 IARS-HEPG2-CRISPR-A304-747A.png<br>Caption: Western blot following CRISPR against IARS in HepG2 whole cell lysate using IARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IARS. IARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV41-33BGHcLV41-34
Sample_IDBGHcLV41-33BGHcLV41-34
Sample Name
Sample NameEIF3K-BGHcLV41-33EIF3K-BGHcLV41-34
RBPEIF3KEIF3K
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1296412965




Library-Prep Information
BGHcLV41-33BGHcLV41-34
Sample #1718
Sample NameEIF3K-BGHcLV41-33EIF3K-BGHcLV41-34
Sample_Name_Alias
Index Well PositionA03B03
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp299296
Peak_Molarity135.0078.90
libSampleQC_DNA_WellDNA_Library_set70/set1/A3.pngDNA_Library_set70/set1/B3.png
RIN9.89.9
libSample_RNA_WellRNA_Library_set70/set1/E8.pngRNA_Library_set70/set1/F8.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-33BGHcLV41-34
RBPEIF3KEIF3K
Batch_IDBGHcLV41BGHcLV41
WB_result68.10080.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42424243




Sequencing Information
BGHcLV41-33BGHcLV41-34
Sample_IDBGHcLV41-33BGHcLV41-34
Sample NameEIF3K-BGHcLV41-33EIF3K-BGHcLV41-34
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads41,469,80534,323,365
total_aligned_reads38,598,98931,724,356
unique_aligned_reads36,506,43729,989,998
percent_uniqueAligned0.880310.87375
correlation_replicates0.9992460.999246
spikein_reads6,0581,188
percent_spikeins0.000150.00003
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29612962




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database