EFL1 RNA-Seq in HepG2

EFL1 CRISPR in HepG2 Control: NT-BGHcLV41-1,NT-BGHcLV41-2

General Information


RBP	EFL1
Cell_Line	HepG2
Method	CRISPR
Exp_Name	EFL1-BGHcLV41-HepG2
ENCODE_series_ID
Batch_ID	BGHcLV41
Pool ID	Pool-240314
Local_Set_Name	set70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1	EFL1-BGHcLV41-23
Rep2	EFL1-BGHcLV41-24
CN1	NT-BGHcLV41-1
CN2	NT-BGHcLV41-2
Rep1_qPCR	41.3
Rep2_qPCR	24.7
Rep1_WB
Rep2_WB
Antibody Cat#	A305-854A-M
Antibody Lot#	lot # 1
Antibody DCC ID
Status	Submitted
Project	ENCORE2
ID	1448

Experiment Information (Status: Submitting)

	BGHcLV41-23	BGHcLV41-24
idx	1290	1291
TRCN#_or_BGC#	BGC#0000954	BGC#0000954
shRNA_or_gRNA_sequence	TATATAGTAATCTCCCCACA	TATATAGTAATCTCCCCACA
PAM	AGG	AGG
Name	EFTUD1_80	EFTUD1_80
Sample_ID	BGHcLV41-23	BGHcLV41-24
transduction_Date	1/12/24	1/12/24
days	D6	D6
RBP_name	EFL1	EFL1
qPCR_result	41.3	24.7
Ave_qPCR	33.0
RT-qPCR_primer-F	cccatatcccatgctgttct
RT-qPCR_primer-R	tgcttgagtttctggtggaa
protein_conc	2974	3598
WB_result
Ave_WB
WB_DONE_date	1/25/24,2/5/24
MW	125KD
IP
antibody_Cat#	A305-854A-M	lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note	check result befor release	check result befor release
ID	9529	9530

Western Blot Western Blot info was not avaliable

Experiment Status

	BGHcLV41-23	BGHcLV41-24
Sample_ID	BGHcLV41-23	BGHcLV41-24
Sample Name
Sample Name	EFL1-BGHcLV41-23	EFL1-BGHcLV41-24
RBP	EFL1	EFL1
Cell_Line	HepG2	HepG2
Exp UID
Status	Submitting	Submitting
Status_date	2024-09-20	2024-09-20
Project	ENCORE2	ENCORE2
Note
ID	12956	12957

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV41-23	BGHcLV41-24
Sample #	9	10
Sample Name	EFL1-BGHcLV41-23	EFL1-BGHcLV41-24
Sample_Name_Alias
Index Well Position	A02	B02
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2024-03-01	2024-03-01
Lib_ID	Lib-240301	Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp	294	293
Peak_Molarity	86.30	94.80
libSampleQC_DNA_Well	DNA_Library_set70/set1/A2.png	DNA_Library_set70/set1/B2.png
RIN	9.8	9.9
libSample_RNA_Well	RNA_Library_set70/set1/E7.png	RNA_Library_set70/set1/F7.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-03-14	2024-03-14
Sample_ID	BGHcLV41-23	BGHcLV41-24
RBP	EFL1	EFL1
Batch_ID	BGHcLV41	BGHcLV41
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4234	4235

Sequencing Information

	BGHcLV41-23	BGHcLV41-24
Sample_ID	BGHcLV41-23	BGHcLV41-24
Sample Name	EFL1-BGHcLV41-23	EFL1-BGHcLV41-24
Pool ID	Pool-240314	Pool-240314
LocalServer_folder	set70	set70
total_reads	27,875,566	36,703,650
total_aligned_reads	23,460,825	33,098,306
unique_aligned_reads	22,112,218	31,216,239
percent_uniqueAligned	0.79325	0.85049
correlation_replicates	0.998663	0.998663
spikein_reads	5,026	1,192
percent_spikeins	0.00018	0.00003
original_ReadLength	151	151
QC_Status	Submitting	Submitting
ID	2953	2954

Data Submission Information DCC submission information was not avaliable

File Information Files of the experiment was not avaliable in the database