IGF2BP1      shRNA in HepG2      Batch: BGHLV12

Experiment Information (Status: NotSatisfied)
BGHLV12-39BGHLV12-40
idx3940
TRCN#_or_BGC#TRCN0000075149TRCN0000075149
shRNA_or_gRNA_sequenceGCAGTGGTGAATGTCACCTATGCAGTGGTGAATGTCACCTAT
PAM
NameIGF2BP1IGF2BP1
Sample_IDBGHLV12-39BGHLV12-40
transduction_Date5/15/20145/15/2014
days
RBP_nameIGF2BP1IGF2BP1
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc00
WB_result
Ave_WBseesee
WB_DONE_date
MW
IP0.5MB0.5MB
antibody_Cat#RN007PRN007P
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
Action
Library_start_date
repeat_library
Note
ID20862087




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
IGF2BP1Product_ID: RN007P
Lot_ID: 3
Source: MBLI
Target Name: IGF2BP1-human		 HepG2_MBLI_RN007P_003_IGF2BP1.png<br>Caption: IP-WB analysis of HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 1% twenty million whole cell lysate input, lane 2 is 1% of the supernatant afer IP and Lane 3 is 10% of IP enrichment using rabbit polyclonal anti-IGF2BP1 Antibody. Lane 4 is 1% ten million whole cell lysate input and lane 5 is 25% IP enrichment using rabbit normal IgG (lanes under 'IgG').
 IGF2BP1_Secondary_Western.png<br>Caption: Western blot following shRNA against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP1. Lanes 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
IGF2BP1_CRISPR.png<br>Caption: Western blot following CRISPR against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IGF2BP1. 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_MBLI_RN007P_003_IGF2BP1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 2.5% of five million whole cell lysate input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-IGF2BP1 antibody (lanes under 'IGF2BP1') or using rabbit normal IgG (lanes under 'IgG').
 IGF2BP1_Secondary_Western.png<br>Caption: Western blot following shRNA against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP1. Lanes 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
IGF2BP1_CRISPR.png<br>Caption: Western blot following CRISPR against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IGF2BP1. 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.




Experiment Status
BGHLV12-39BGHLV12-40
Sample_IDBGHLV12-39BGHLV12-40
Sample Name
Sample Name
RBPIGF2BP1IGF2BP1
Cell_LineHepG2HepG2
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2019-06-262019-06-26
ProjectENCODE3ENCODE3
Note
ID18371838




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database