STAU2 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-81	BG293LV06-82
idx	0	0
TRCN#_or_BGC#	TRCN0000157149	TRCN0000157149
shRNA_or_gRNA_sequence	CCGGGCCAGGGAACTCCTTATGAATCTCGAGATTCATAAGGAGTTCCCTGGCTTTTTTG	CCGGGCCAGGGAACTCCTTATGAATCTCGAGATTCATAAGGAGTTCCCTGGCTTTTTTG
PAM
Name	STAU2	STAU2
Sample_ID	BG293LV06-81	BG293LV06-82
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	STAU2	STAU2
qPCR_result	75.8	86.7
Ave_qPCR	81.2
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12216	12217

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-81	BG293LV06-82
Sample_ID	BG293LV06-81	BG293LV06-82
Sample Name
Sample Name	STAU2-BG293LV06-81B	STAU2-BG293LV06-82B
RBP	STAU2	STAU2
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13518	13519

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-81	BG293LV06-82
Sample #	81	82
Sample Name	STAU2-BG293LV06-81B	STAU2-BG293LV06-82B
Sample_Name_Alias
Index Well Position	A04	B04
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-10-02	2024-10-02
Lib_ID	Lib-241002	Lib-241002
Tecan_Location
Tecan
Tecan_date
Size_bp	297	286
Peak_Molarity	82.60	96.50
libSampleQC_DNA_Well	DNA_Library_set72/set1/A4.png	DNA_Library_set72/set1/B4.png
RIN	9.9	9.9
libSample_RNA_Well	RNA_Library_set72/set1/A4.png	RNA_Library_set72/set1/B4.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-10-04	2024-10-04
Sample_ID	BG293LV06-81	BG293LV06-82
RBP	STAU2	STAU2
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix	B	B
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4506	4507

Sequencing Information

	BG293LV06-81	BG293LV06-82
Sample_ID	BG293LV06-81	BG293LV06-82
Sample Name	STAU2-BG293LV06-81B	STAU2-BG293LV06-82B
Pool ID	Pool-241004	Pool-241004
LocalServer_folder	set72	set72
total_reads	35,170,400	22,564,208
total_aligned_reads	32,307,806	20,918,733
unique_aligned_reads	30,151,295	19,664,244
percent_uniqueAligned	0.85729	0.87148
correlation_replicates	0.965510	0.965510
spikein_reads	151,914	80,238
percent_spikeins	0.00432	0.00356
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3075	3076

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
STAU2-BG293LV06-81B_S33_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3075	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29816
STAU2-BG293LV06-81B_S33_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3075	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29888
STAU2-BG293LV06-81B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3075					GRCh38	V40	STAU2-BG293LV06-81B_S33_L001_R1_001.filtered.trimmed.paired.fastq.gz,STAU2-BG293LV06-81B_S33_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29960
STAU2-BG293LV06-81B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3075					GRCh38	V40	STAU2-BG293LV06-81B_Aligned.sortedByCoord.out.bam		ENCORE2	30032
STAU2-BG293LV06-81B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3075					GRCh38	V40	STAU2-BG293LV06-81B_Aligned.sortedByCoord.out.bam		ENCORE2	30104
STAU2-BG293LV06-81B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3075					GRCh38	V40	STAU2-BG293LV06-81B_Aligned.sortedByCoord.out.bam		ENCORE2	30176
STAU2-BG293LV06-81B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3075					GRCh38	V40	STAU2-BG293LV06-81B_Aligned.sortedByCoord.out.bam		ENCORE2	30248
STAU2-BG293LV06-81B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3075					GRCh38	V40	STAU2-BG293LV06-81B_S33_L001_R1_001.filtered.trimmed.paired.fastq.gz,STAU2-BG293LV06-81B_S33_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30320
STAU2-BG293LV06-82B_S34_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3076	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29817
STAU2-BG293LV06-82B_S34_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3076	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29889
STAU2-BG293LV06-82B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3076					GRCh38	V40	STAU2-BG293LV06-82B_S34_L001_R1_001.filtered.trimmed.paired.fastq.gz,STAU2-BG293LV06-82B_S34_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29961
STAU2-BG293LV06-82B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3076					GRCh38	V40	STAU2-BG293LV06-82B_Aligned.sortedByCoord.out.bam		ENCORE2	30033
STAU2-BG293LV06-82B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3076					GRCh38	V40	STAU2-BG293LV06-82B_Aligned.sortedByCoord.out.bam		ENCORE2	30105
STAU2-BG293LV06-82B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3076					GRCh38	V40	STAU2-BG293LV06-82B_Aligned.sortedByCoord.out.bam		ENCORE2	30177
STAU2-BG293LV06-82B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3076					GRCh38	V40	STAU2-BG293LV06-82B_Aligned.sortedByCoord.out.bam		ENCORE2	30249
STAU2-BG293LV06-82B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3076					GRCh38	V40	STAU2-BG293LV06-82B_S34_L001_R1_001.filtered.trimmed.paired.fastq.gz,STAU2-BG293LV06-82B_S34_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30321