RBM14 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-29	BG293LV06-30
idx	0	0
TRCN#_or_BGC#	TRCN0000072693	TRCN0000072693
shRNA_or_gRNA_sequence	CCGGCCCAGGAGATGATCCTGTTAACTCGAGTTAACAGGATCATCTCCTGGGTTTTTG	CCGGCCCAGGAGATGATCCTGTTAACTCGAGTTAACAGGATCATCTCCTGGGTTTTTG
PAM
Name	RBM14	RBM14
Sample_ID	BG293LV06-29	BG293LV06-30
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	RBM14	RBM14
qPCR_result	65.8	66.6
Ave_qPCR	66.2
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12164	12165

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-29	BG293LV06-30
Sample_ID	BG293LV06-29	BG293LV06-30
Sample Name
Sample Name	RBM14-BG293LV06-29	RBM14-BG293LV06-30
RBP	RBM14	RBM14
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13466	13467

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-29	BG293LV06-30
Sample #	33	34
Sample Name	RBM14-BG293LV06-29	RBM14-BG293LV06-30
Sample_Name_Alias
Index Well Position	A05	B05
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-09-25	2024-09-25
Lib_ID	Lib-240925B	Lib-240925B
Tecan_Location
Tecan
Tecan_date
Size_bp	271	279
Peak_Molarity	74.80	106.00
libSampleQC_DNA_Well	DNA_Library_set71/set1/A5.png	DNA_Library_set71/set1/B5.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set71/set1/A5.png	RNA_Library_set71/set1/B5.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-09-30	2024-09-30
Sample_ID	BG293LV06-29	BG293LV06-30
RBP	RBM14	RBM14
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4458	4459

Sequencing Information

	BG293LV06-29	BG293LV06-30
Sample_ID	BG293LV06-29	BG293LV06-30
Sample Name	RBM14-BG293LV06-29	RBM14-BG293LV06-30
Pool ID	Pool-240930	Pool-240930
LocalServer_folder	set71	set71
total_reads	31,744,250	26,283,782
total_aligned_reads	28,496,697	23,597,903
unique_aligned_reads	26,583,906	22,131,162
percent_uniqueAligned	0.83744	0.84201
correlation_replicates	0.967588	0.967588
spikein_reads	142,030	80,956
percent_spikeins	0.00447	0.00308
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3027	3028

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RBM14-BG293LV06-29_S33_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3027	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29361
RBM14-BG293LV06-29_S33_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3027	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29425
RBM14-BG293LV06-29_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3027					GRCh38	V40	RBM14-BG293LV06-29_S33_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM14-BG293LV06-29_S33_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29490
RBM14-BG293LV06-29_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3027					GRCh38	V40	RBM14-BG293LV06-29_Aligned.sortedByCoord.out.bam		ENCORE2	29554
RBM14-BG293LV06-29_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3027					GRCh38	V40	RBM14-BG293LV06-29_Aligned.sortedByCoord.out.bam		ENCORE2	29618
RBM14-BG293LV06-29_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3027					GRCh38	V40	RBM14-BG293LV06-29_Aligned.sortedByCoord.out.bam		ENCORE2	29682
RBM14-BG293LV06-29_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3027					GRCh38	V40	RBM14-BG293LV06-29_S33_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM14-BG293LV06-29_S33_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29746
RBM14-BG293LV06-30_S34_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3028	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29362
RBM14-BG293LV06-30_S34_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3028	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29426
RBM14-BG293LV06-30_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3028					GRCh38	V40	RBM14-BG293LV06-30_S34_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM14-BG293LV06-30_S34_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29491
RBM14-BG293LV06-30_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3028					GRCh38	V40	RBM14-BG293LV06-30_Aligned.sortedByCoord.out.bam		ENCORE2	29555
RBM14-BG293LV06-30_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3028					GRCh38	V40	RBM14-BG293LV06-30_Aligned.sortedByCoord.out.bam		ENCORE2	29619
RBM14-BG293LV06-30_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3028					GRCh38	V40	RBM14-BG293LV06-30_Aligned.sortedByCoord.out.bam		ENCORE2	29683
RBM14-BG293LV06-30_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3028					GRCh38	V40	RBM14-BG293LV06-30_S34_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM14-BG293LV06-30_S34_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29747