HNRNPH1 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-125	BG293LV06-126
idx	0	0
TRCN#_or_BGC#	TRCN0000074596	TRCN0000074596
shRNA_or_gRNA_sequence	CCGGCCTGTGAGAGTACACATTGAACTCGAGTTCAATGTGTACTCTCACAGGTTTTTG	CCGGCCTGTGAGAGTACACATTGAACTCGAGTTCAATGTGTACTCTCACAGGTTTTTG
PAM
Name	HNRNPH1	HNRNPH1
Sample_ID	BG293LV06-125	BG293LV06-126
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	HNRNPH1	HNRNPH1
qPCR_result	90.1	90.9
Ave_qPCR	90.5
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12260	12261

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-125	BG293LV06-126
Sample_ID	BG293LV06-125	BG293LV06-126
Sample Name
Sample Name	HNRNPH1-BG293LV06-125B	HNRNPH1-BG293LV06-126B
RBP	HNRNPH1	HNRNPH1
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13562	13563

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-125	BG293LV06-126
Sample #	125	126
Sample Name	HNRNPH1-BG293LV06-125B	HNRNPH1-BG293LV06-126B
Sample_Name_Alias
Index Well Position	E09	F09
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-10-02	2024-10-02
Lib_ID	Lib-241002	Lib-241002
Tecan_Location
Tecan
Tecan_date
Size_bp	283	279
Peak_Molarity	90.90	92.00
libSampleQC_DNA_Well	DNA_Library_set72/set1/E9.png	DNA_Library_set72/set1/F9.png
RIN	9.9	10.0
libSample_RNA_Well	RNA_Library_set72/set1/E9.png	RNA_Library_set72/set1/F9.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-10-04	2024-10-04
Sample_ID	BG293LV06-125	BG293LV06-126
RBP	HNRNPH1	HNRNPH1
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix	B	B
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4550	4551

Sequencing Information

	BG293LV06-125	BG293LV06-126
Sample_ID	BG293LV06-125	BG293LV06-126
Sample Name	HNRNPH1-BG293LV06-125B	HNRNPH1-BG293LV06-126B
Pool ID	Pool-241004	Pool-241004
LocalServer_folder	set72	set72
total_reads	35,039,324	35,429,325
total_aligned_reads	33,353,510	33,605,474
unique_aligned_reads	31,475,018	31,573,832
percent_uniqueAligned	0.89828	0.89118
correlation_replicates	0.996495	0.996495
spikein_reads	104,830	82,450
percent_spikeins	0.00299	0.00233
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3119	3120

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
HNRNPH1-BG293LV06-125B_S77_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3119	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29782
HNRNPH1-BG293LV06-125B_S77_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3119	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29854
HNRNPH1-BG293LV06-125B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3119					GRCh38	V40	HNRNPH1-BG293LV06-125B_S77_L001_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH1-BG293LV06-125B_S77_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29926
HNRNPH1-BG293LV06-125B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3119					GRCh38	V40	HNRNPH1-BG293LV06-125B_Aligned.sortedByCoord.out.bam		ENCORE2	29998
HNRNPH1-BG293LV06-125B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3119					GRCh38	V40	HNRNPH1-BG293LV06-125B_Aligned.sortedByCoord.out.bam		ENCORE2	30070
HNRNPH1-BG293LV06-125B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3119					GRCh38	V40	HNRNPH1-BG293LV06-125B_Aligned.sortedByCoord.out.bam		ENCORE2	30142
HNRNPH1-BG293LV06-125B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3119					GRCh38	V40	HNRNPH1-BG293LV06-125B_Aligned.sortedByCoord.out.bam		ENCORE2	30214
HNRNPH1-BG293LV06-125B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3119					GRCh38	V40	HNRNPH1-BG293LV06-125B_S77_L001_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH1-BG293LV06-125B_S77_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30286
HNRNPH1-BG293LV06-126B_S78_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3120	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29783
HNRNPH1-BG293LV06-126B_S78_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3120	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29855
HNRNPH1-BG293LV06-126B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3120					GRCh38	V40	HNRNPH1-BG293LV06-126B_S78_L001_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH1-BG293LV06-126B_S78_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29927
HNRNPH1-BG293LV06-126B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3120					GRCh38	V40	HNRNPH1-BG293LV06-126B_Aligned.sortedByCoord.out.bam		ENCORE2	29999
HNRNPH1-BG293LV06-126B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3120					GRCh38	V40	HNRNPH1-BG293LV06-126B_Aligned.sortedByCoord.out.bam		ENCORE2	30071
HNRNPH1-BG293LV06-126B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3120					GRCh38	V40	HNRNPH1-BG293LV06-126B_Aligned.sortedByCoord.out.bam		ENCORE2	30143
HNRNPH1-BG293LV06-126B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3120					GRCh38	V40	HNRNPH1-BG293LV06-126B_Aligned.sortedByCoord.out.bam		ENCORE2	30215
HNRNPH1-BG293LV06-126B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3120					GRCh38	V40	HNRNPH1-BG293LV06-126B_S78_L001_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH1-BG293LV06-126B_S78_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30287