TIAL1 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-111	BG293LV06-112
idx	0	0
TRCN#_or_BGC#	TRCN0000017211	TRCN0000017211
shRNA_or_gRNA_sequence	CGGATATGGTATGGCAAGTTA	CGGATATGGTATGGCAAGTTA
PAM
Name	TIAL1	TIAL1
Sample_ID	BG293LV06-111	BG293LV06-112
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	TIAL1	TIAL1
qPCR_result	48.2	53.9
Ave_qPCR	51.0
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12246	12247

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-111	BG293LV06-112
Sample_ID	BG293LV06-111	BG293LV06-112
Sample Name
Sample Name	TIAL1-BG293LV06-111B	TIAL1-BG293LV06-112B
RBP	TIAL1	TIAL1
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13548	13549

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-111	BG293LV06-112
Sample #	111	112
Sample Name	TIAL1-BG293LV06-111B	TIAL1-BG293LV06-112B
Sample_Name_Alias
Index Well Position	G07	H07
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-10-02	2024-10-02
Lib_ID	Lib-241002	Lib-241002
Tecan_Location
Tecan
Tecan_date
Size_bp	264	270
Peak_Molarity	77.60	55.60
libSampleQC_DNA_Well	DNA_Library_set72/set1/G7.png	DNA_Library_set72/set1/H7.png
RIN	9.8	9.9
libSample_RNA_Well	RNA_Library_set72/set1/G7.png	RNA_Library_set72/set1/H7.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-10-04	2024-10-04
Sample_ID	BG293LV06-111	BG293LV06-112
RBP	TIAL1	TIAL1
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix	B	B
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4536	4537

Sequencing Information

	BG293LV06-111	BG293LV06-112
Sample_ID	BG293LV06-111	BG293LV06-112
Sample Name	TIAL1-BG293LV06-111B	TIAL1-BG293LV06-112B
Pool ID	Pool-241004	Pool-241004
LocalServer_folder	set72	set72
total_reads	51,768,968	32,134,758
total_aligned_reads	47,821,182	29,431,879
unique_aligned_reads	44,810,244	27,533,893
percent_uniqueAligned	0.86558	0.85683
correlation_replicates	0.994590	0.994590
spikein_reads	126,784	97,380
percent_spikeins	0.00245	0.00303
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3105	3106

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
TIAL1-BG293LV06-111B_S63_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3105	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29818
TIAL1-BG293LV06-111B_S63_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3105	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29890
TIAL1-BG293LV06-111B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3105					GRCh38	V40	TIAL1-BG293LV06-111B_S63_L001_R1_001.filtered.trimmed.paired.fastq.gz,TIAL1-BG293LV06-111B_S63_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29962
TIAL1-BG293LV06-111B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3105					GRCh38	V40	TIAL1-BG293LV06-111B_Aligned.sortedByCoord.out.bam		ENCORE2	30034
TIAL1-BG293LV06-111B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3105					GRCh38	V40	TIAL1-BG293LV06-111B_Aligned.sortedByCoord.out.bam		ENCORE2	30106
TIAL1-BG293LV06-111B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3105					GRCh38	V40	TIAL1-BG293LV06-111B_Aligned.sortedByCoord.out.bam		ENCORE2	30178
TIAL1-BG293LV06-111B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3105					GRCh38	V40	TIAL1-BG293LV06-111B_Aligned.sortedByCoord.out.bam		ENCORE2	30250
TIAL1-BG293LV06-111B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3105					GRCh38	V40	TIAL1-BG293LV06-111B_S63_L001_R1_001.filtered.trimmed.paired.fastq.gz,TIAL1-BG293LV06-111B_S63_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30322
TIAL1-BG293LV06-112B_S64_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3106	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29819
TIAL1-BG293LV06-112B_S64_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3106	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29891
TIAL1-BG293LV06-112B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3106					GRCh38	V40	TIAL1-BG293LV06-112B_S64_L001_R1_001.filtered.trimmed.paired.fastq.gz,TIAL1-BG293LV06-112B_S64_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29963
TIAL1-BG293LV06-112B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3106					GRCh38	V40	TIAL1-BG293LV06-112B_Aligned.sortedByCoord.out.bam		ENCORE2	30035
TIAL1-BG293LV06-112B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3106					GRCh38	V40	TIAL1-BG293LV06-112B_Aligned.sortedByCoord.out.bam		ENCORE2	30107
TIAL1-BG293LV06-112B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3106					GRCh38	V40	TIAL1-BG293LV06-112B_Aligned.sortedByCoord.out.bam		ENCORE2	30179
TIAL1-BG293LV06-112B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3106					GRCh38	V40	TIAL1-BG293LV06-112B_Aligned.sortedByCoord.out.bam		ENCORE2	30251
TIAL1-BG293LV06-112B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3106					GRCh38	V40	TIAL1-BG293LV06-112B_S64_L001_R1_001.filtered.trimmed.paired.fastq.gz,TIAL1-BG293LV06-112B_S64_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30323