LGALS1 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-101	BG293LV06-102
idx	0	0
TRCN#_or_BGC#	TRCN0000057423	TRCN0000057423
shRNA_or_gRNA_sequence	CCGGGCTGCCAGATGGATACGAATTCTCGAGAATTCGTATCCATCTGGCAGCTTTTTG	CCGGGCTGCCAGATGGATACGAATTCTCGAGAATTCGTATCCATCTGGCAGCTTTTTG
PAM
Name	LGALS1	LGALS1
Sample_ID	BG293LV06-101	BG293LV06-102
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	LGALS1	LGALS1
qPCR_result	97.8	99.1
Ave_qPCR	98.4
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12236	12237

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-101	BG293LV06-102
Sample_ID	BG293LV06-101	BG293LV06-102
Sample Name
Sample Name	LGALS1-BG293LV06-101B	LGALS1-BG293LV06-102B
RBP	LGALS1	LGALS1
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13538	13539

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-101	BG293LV06-102
Sample #	101	102
Sample Name	LGALS1-BG293LV06-101B	LGALS1-BG293LV06-102B
Sample_Name_Alias
Index Well Position	E06	F06
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-10-02	2024-10-02
Lib_ID	Lib-241002	Lib-241002
Tecan_Location
Tecan
Tecan_date
Size_bp	281	283
Peak_Molarity	59.80	80.00
libSampleQC_DNA_Well	DNA_Library_set72/set1/E6.png	DNA_Library_set72/set1/F6.png
RIN	9.8	9.9
libSample_RNA_Well	RNA_Library_set72/set1/E6.png	RNA_Library_set72/set1/F6.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-10-04	2024-10-04
Sample_ID	BG293LV06-101	BG293LV06-102
RBP	LGALS1	LGALS1
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix	B	B
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4526	4527

Sequencing Information

	BG293LV06-101	BG293LV06-102
Sample_ID	BG293LV06-101	BG293LV06-102
Sample Name	LGALS1-BG293LV06-101B	LGALS1-BG293LV06-102B
Pool ID	Pool-241004	Pool-241004
LocalServer_folder	set72	set72
total_reads	49,380,355	51,063,197
total_aligned_reads	46,299,536	47,490,550
unique_aligned_reads	43,270,772	44,442,008
percent_uniqueAligned	0.87628	0.87033
correlation_replicates	0.997570	0.997570
spikein_reads	110,100	131,348
percent_spikeins	0.00223	0.00257
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3095	3096

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
LGALS1-BG293LV06-101B_S53_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3095	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29786
LGALS1-BG293LV06-101B_S53_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3095	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29858
LGALS1-BG293LV06-101B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3095					GRCh38	V40	LGALS1-BG293LV06-101B_S53_L001_R1_001.filtered.trimmed.paired.fastq.gz,LGALS1-BG293LV06-101B_S53_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29930
LGALS1-BG293LV06-101B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3095					GRCh38	V40	LGALS1-BG293LV06-101B_Aligned.sortedByCoord.out.bam		ENCORE2	30002
LGALS1-BG293LV06-101B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3095					GRCh38	V40	LGALS1-BG293LV06-101B_Aligned.sortedByCoord.out.bam		ENCORE2	30074
LGALS1-BG293LV06-101B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3095					GRCh38	V40	LGALS1-BG293LV06-101B_Aligned.sortedByCoord.out.bam		ENCORE2	30146
LGALS1-BG293LV06-101B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3095					GRCh38	V40	LGALS1-BG293LV06-101B_Aligned.sortedByCoord.out.bam		ENCORE2	30218
LGALS1-BG293LV06-101B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3095					GRCh38	V40	LGALS1-BG293LV06-101B_S53_L001_R1_001.filtered.trimmed.paired.fastq.gz,LGALS1-BG293LV06-101B_S53_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30290
LGALS1-BG293LV06-102B_S54_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3096	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29787
LGALS1-BG293LV06-102B_S54_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3096	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29859
LGALS1-BG293LV06-102B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3096					GRCh38	V40	LGALS1-BG293LV06-102B_S54_L001_R1_001.filtered.trimmed.paired.fastq.gz,LGALS1-BG293LV06-102B_S54_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29931
LGALS1-BG293LV06-102B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3096					GRCh38	V40	LGALS1-BG293LV06-102B_Aligned.sortedByCoord.out.bam		ENCORE2	30003
LGALS1-BG293LV06-102B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3096					GRCh38	V40	LGALS1-BG293LV06-102B_Aligned.sortedByCoord.out.bam		ENCORE2	30075
LGALS1-BG293LV06-102B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3096					GRCh38	V40	LGALS1-BG293LV06-102B_Aligned.sortedByCoord.out.bam		ENCORE2	30147
LGALS1-BG293LV06-102B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3096					GRCh38	V40	LGALS1-BG293LV06-102B_Aligned.sortedByCoord.out.bam		ENCORE2	30219
LGALS1-BG293LV06-102B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3096					GRCh38	V40	LGALS1-BG293LV06-102B_S54_L001_R1_001.filtered.trimmed.paired.fastq.gz,LGALS1-BG293LV06-102B_S54_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30291