NSD2

General Information

Gene Name       : NSD2
Gene ID             : ENSG00000109685.17
Essential Gene : No

Functions

Localization

Domains (AlphaFold: NSD2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878NSD2ENSG00000109685.1752.6657.8455.25ENCSR000AEF
HepG2NSD2ENSG00000109685.1746.6549.1447.89ENCSR985KAT
K562NSD2ENSG00000109685.1745.3349.8447.59ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
NSD265127S1Submitted, ENCORE2BGKcLV44-39, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
NSD2K562CRISPRBGKcLV37-19,BGKcLV37-20BGKcLV37-1,BGKcLV37-2no clear band on the position,NotSatisfiedENCORE2
NSD2K562CRISPRBGKcLV44-37,BGKcLV44-38BGKcLV44-1,BGKcLV44-257.5,59.9NotSatisfiedENCORE2
NSD2K562CRISPRNSD2-BGKcLV44-39,NSD2-BGKcLV44-40NT-BGKcLV44-1,NT-BGKcLV44-297.1,77.0SubmittingENCORE2
NSD2HepG2CRISPRBGHcLV36-15,BGHcLV36-16BGHcLV36-1,BGHcLV36-2NO CLEAR BAND ON THE POSITION,NotSatisfiedENCORE2
NSD2HepG2CRISPRBGHcLV45-47,BGHcLV45-48BGHcLV45-1,BGHcLV45-20.0,0.0NotSatisfiedENCORE2
NSD2HepG2CRISPRBGHcLV50-71,BGHcLV50-72BGHcLV50-1,BGHcLV50-267.2,63.5NotSatisfiedENCORE2
NSD2HepG2CRISPRBGHcLV50-73,BGHcLV50-74BGHcLV50-1,BGHcLV50-20.0,0.0NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NSD2Product_ID: 65127S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: NSD2-human		 CST_65127S_1_NSD2.png<br>Caption: IP-WB analysis of 65127S whole cell lysate using the NSD2 specific antibody, 65127S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the NSD2-specific antibody, 65127S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NSD2-K562-CRISPR-65127S.png<br>Caption: Western blot following CRISPR against NSD2 in K562 whole cell lysate using NSD2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NSD2. NSD2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for NSD2 is not avaliable on ENCORE database.



Proteins bound to RNA

  • NO known proteins bind on NSD2 RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for NSD2 is not avaliable on ENCORE database.






NO known proteins are bound to NSD2 RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for NSD2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for NSD2 is not available on ENCORE database.

  • No RBPs affect the gene expression of NSD2 on ENCORE database.


The Knockdown/knockout RNA-Seq assay for NSD2 is not available on ENCORE database.






No RBPs affect the gene expression of NSD2 on ENCORE database.






The Knockdown/knockout RNA-Seq assay for NSD2 is not available on ENCORE database.






No RBPs affect the alternative splcing events of NSD2 on ENCORE database.





The Knockdown/knockout RNA-Seq assay for NSD2 is not avaliable on ENCORE database.