HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PRPF4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF4 antibody (lanes under 'PRPF4'). Method: immunoprecipitation Releated Sample: eCLIP:752 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against PRPF4 in HepG2 whole cell lysate using PRPF4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PRPF4.PRPF4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGHLV37-41 Status: Released Lab: Graveley Lab |
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K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using PRPF4 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-PRPF4 antibody (lanes under 'anti-PRPF4'). Method: immunoprecipitation Status: Released Lab: Fu Lab | 
Caption: Western blot following shRNA against PRPF4 in k562 whole cell lysate using PRPF4 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PRPF4.PRPF4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKLV38-63 Status: Released Lab: Graveley Lab |
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