| HepG2 | | 
Caption: Western blot following shRNA against AGO1/EIF2C1 in HepG2 whole cell lysate using EIF2C1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2C1. EIF2C1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGHLV14-13 Status: Released Lab: Graveley Lab |
|---|
| K562 | 
Caption: IP-WB characterization of AGO1 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-EIF2C1(AGO1)(Human)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Method: immunoprecipitation Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against EIF2C1 in K562 whole cell lysate using EIF2C1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2C1. EIF2C1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKLV13-69 Status: Released Lab: Graveley Lab 
Caption: Western blot following shRNA against AGO1 in K562 whole cell lysate using AGO1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against AGO1. AGO1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow. Releated Sample: BGKLV13-69 Status: NotSatisfied Lab: Graveley Lab |
|---|