| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FIP1L1 specific antibody. Lane 1 is 0.5% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 5% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 5% IP enrichment using rabbit polyclonal anti-FIP1L1 antibody (lanes under 'anti-FIP1L1'). Asterisk indicates heavy chain of antibody. Method: immunoprecipitation Status: Released Lab: Fu Lab | 
Caption: Western blot following shRNA against FIP1L1 in K562 and HepG2 whole cell lysate using FIP1L1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FIP1L1. Lanes 5-8 follow the same pattern, but in HepG2. FIP1L1 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV29-33 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using FIP1L1 specific antibody. Lane 1 is 0.5% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 5% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 5% IP enrichment using rabbit polyclonal anti-FIP1L1 antibody (lanes under 'anti-FIP1L1'). Asterisk indicates heavy chain of antibody. Method: immunoprecipitation Status: Released Lab: Fu Lab |
Caption: Western blot following shRNA against FIP1L1 in K562 and HepG2 whole cell lysate using FIP1L1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FIP1L1. Lanes 5-8 follow the same pattern, but in HepG2. FIP1L1 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV29-33 Status: Released Lab: Graveley Lab |
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