EXOSC9 antibody: A303-888A


Antibody Info
Product_IDA303-888A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB573YWT
Target Info
GeneNameEXOSC9
Phosphate
Target_nameEXOSC9-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 EXOSC9_Secondary_Western.png<br>Caption: Western blot following shRNA against EXOSC9 in K562 and HepG2 whole cell lysate using EXOSC9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EXOSC9. Lanes 5-7 follow the same pattern, but in HepG2. EXOSC9 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against EXOSC9 in K562 and HepG2 whole cell lysate using EXOSC9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EXOSC9. Lanes 5-7 follow the same pattern, but in HepG2. EXOSC9 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV13-7

Status: Released

Lab: Graveley Lab

EXOSC9-HEPG2-CRISPR-A303-888A.png<br>Caption: Western blot following CRISPR against EXOSC9 in HepG2 whole cell lysate using EXOSC9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EXOSC9. EXOSC9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against EXOSC9 in HepG2 whole cell lysate using EXOSC9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EXOSC9. EXOSC9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHcLV41-59

Status: Submitted

Lab: Graveley Lab

K562 K562_Bethyl_A303-888A_1_EXOSC9.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PRPF8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF8 antibody (lanes under 'PRPF8').

Caption: IP-Western Blot analysis of K562 whole cell lysate using PRPF8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF8 antibody (lanes under 'PRPF8').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

EXOSC9_Secondary_Western.png<br>Caption: Western blot following shRNA against EXOSC9 in K562 and HepG2 whole cell lysate using EXOSC9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EXOSC9. Lanes 5-7 follow the same pattern, but in HepG2. EXOSC9 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against EXOSC9 in K562 and HepG2 whole cell lysate using EXOSC9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EXOSC9. Lanes 5-7 follow the same pattern, but in HepG2. EXOSC9 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV13-7

Status: Released

Lab: Graveley Lab

EXOSC9-K562-CRISPR-A303-888A.png<br>Caption: Western blot following CRISPR against EXOSC9 in K562 whole cell lysate using EXOSC9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EXOSC9. EXOSC9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against EXOSC9 in K562 whole cell lysate using EXOSC9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EXOSC9. EXOSC9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGKcLV40-39

Status: Submitted

Lab: Graveley Lab