SF1 antibody: A303-213A


Antibody Info
Product_IDA303-213A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB539EUO
Target Info
GeneNameSF1
Phosphate
Target_nameSF1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 SF1-HEPG2-A303-213A.png<br>Caption: Western blot following shRNA against SF1 in HepG2 whole cell lysate using SF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SF1. SF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following shRNA against SF1 in HepG2 whole cell lysate using SF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SF1. SF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHLV26-51

Status: Experimented

Lab: Graveley Lab

K562 Bethyl_A303-213A_1_SF1.png<br>Caption: Immunoprecipitation lane (3) exhibits a large deviation from the expected protein size, which we believe is due to performing the characterization using the Jess Western Blotting system. This system has a documented tendency for some proteins to run differently than with more traditional methods due to their compositions and interactions with the electrophoretic components. The image also closely resembles the example image provided by the vendor but secondary validation will be needed for verification.

Caption: Immunoprecipitation lane (3) exhibits a large deviation from the expected protein size, which we believe is due to performing the characterization using the Jess Western Blotting system. This system has a documented tendency for some proteins to run differently than with more traditional methods due to their compositions and interactions with the electrophoretic components. The image also closely resembles the example image provided by the vendor but secondary validation will be needed for verification.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab