DGCR8 antibody: A302-468A


Antibody Info
Product_IDA302-468A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB462ILE
Target Info
GeneNameDGCR8
Phosphate
Target_nameDGCR8-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A302-468A_1_DGCR8.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DGCR8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DGCR8 antibody (lanes under 'DGCR8').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DGCR8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DGCR8 antibody (lanes under 'DGCR8').

Method: immunoprecipitation

Releated Sample: eCLIP:383

Status: Released

Lab: Yeo Lab

K562 K562_Bethyl_A302-468A_1_DGCR8.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DGCR8 antibody (lanes under 'DGCR8').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DGCR8 antibody (lanes under 'DGCR8').

Method: immunoprecipitation

Releated Sample: eCLIP:341

Status: Released

Lab: Yeo Lab

DGCR8-K562-D22-CRISPR.png<br>Caption: Western blot following CRISPR against DGCR8 in K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DGCR8. DGCR8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. This western blot was done 22 days after the CRISPR experiment process.

Caption: Western blot following CRISPR against DGCR8 in K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DGCR8. DGCR8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. This western blot was done 22 days after the CRISPR experiment process.

Releated Sample: BGKcLV02-19-D22

Status: Released

Lab: Graveley Lab

DGCR8-K562-D41-CRISPR.png<br>Caption: Western blot following CRISPR against DGCR8 in K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DGCR8. DGCR8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. This western blot was done 41 days after the CRISPR experiment process.

Caption: Western blot following CRISPR against DGCR8 in K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DGCR8. DGCR8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. This western blot was done 41 days after the CRISPR experiment process.

Releated Sample: BGKcLV02-19-D41

Status: Released

Lab: Graveley Lab

DGCR8-K562-D30-CRISPR.png<br>Caption: Western blot following CRISPR against DGCR8 in K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DGCR8. DGCR8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. This western blot was done 30 days after the CRISPR experiment process.

Caption: Western blot following CRISPR against DGCR8 in K562 whole cell lysate using DGCR8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DGCR8. DGCR8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. This western blot was done 30 days after the CRISPR experiment process.

Releated Sample: BGKcLV02-19-D30

Status: Released

Lab: Graveley Lab