DHX30 antibody: A302-218A


Antibody Info
Product_IDA302-218A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB596PUY
Target Info
GeneNameDHX30
Phosphate
Target_nameDHX30-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A302-218A_1_DHX30.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DHX30 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DHX30 antibody (lanes under 'DHX30').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DHX30 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DHX30 antibody (lanes under 'DHX30').

Method: immunoprecipitation

Releated Sample: eCLIP:654

Status: Released

Lab: Yeo Lab

DHX30_Secondary_Western.png<br>Caption: Western blot following shRNA against DHX30 in K562 and HepG2 whole cell lysate using DHX30 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DHX30. Lanes 5-8 follow the same pattern, but in HepG2. DHX30 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against DHX30 in K562 and HepG2 whole cell lysate using DHX30 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DHX30. Lanes 5-8 follow the same pattern, but in HepG2. DHX30 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV24-15

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A302-218A_1_DHX30.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DHX30 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DHX30 antibody (lanes under 'DHX30').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DHX30 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DHX30 antibody (lanes under 'DHX30').

Method: immunoprecipitation

Releated Sample: eCLIP:682

Status: Released

Lab: Yeo Lab

DHX30_Secondary_Western.png<br>Caption: Western blot following shRNA against DHX30 in K562 and HepG2 whole cell lysate using DHX30 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DHX30. Lanes 5-8 follow the same pattern, but in HepG2. DHX30 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against DHX30 in K562 and HepG2 whole cell lysate using DHX30 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DHX30. Lanes 5-8 follow the same pattern, but in HepG2. DHX30 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV24-15

Status: Released

Lab: Graveley Lab