GRWD1 antibody: A301-576A


Antibody Info
Product_IDA301-576A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB573ZJV
Target Info
GeneNameGRWD1
Phosphate
Target_nameGRWD1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-576A_1_GRWD1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GRWD1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GRWD1 antibody (lanes under 'GRWD1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GRWD1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GRWD1 antibody (lanes under 'GRWD1').

Method: immunoprecipitation

Releated Sample: eCLIP:628

Status: Released

Lab: Yeo Lab

GRWD1_Secondary_Western.png<br>Caption: Western blot following shRNA against GRWD1 in K562 and HepG2 whole cell lysate using GRWD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against GRWD1. Lanes 5-7 follow the same pattern, but in HepG2. GRWD1 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against GRWD1 in K562 and HepG2 whole cell lysate using GRWD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against GRWD1. Lanes 5-7 follow the same pattern, but in HepG2. GRWD1 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV21-15

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-576A_1_GRWD1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using GRWD1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GRWD1 antibody (lanes under 'GRWD1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using GRWD1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GRWD1 antibody (lanes under 'GRWD1').

Method: immunoprecipitation

Releated Sample: eCLIP:645

Status: Released

Lab: Yeo Lab

GRWD1_Secondary_Western.png<br>Caption: Western blot following shRNA against GRWD1 in K562 and HepG2 whole cell lysate using GRWD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against GRWD1. Lanes 5-7 follow the same pattern, but in HepG2. GRWD1 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against GRWD1 in K562 and HepG2 whole cell lysate using GRWD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against GRWD1. Lanes 5-7 follow the same pattern, but in HepG2. GRWD1 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV21-15

Status: Released

Lab: Graveley Lab