INTS11 antibody: A301-274A


Antibody Info
Product_IDA301-274A
Lot_ID4
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB499MQV
Target Info
GeneNameCPSF3L
Phosphate
Target_nameCPSF3L-human
Antibody Info
Product_IDA301-274A
Lot_ID4
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB499MQV
Target Info
GeneNameINTS11
Phosphate
Target_nameINTS11-human

Validation Images
Cell linePrimary validationSecondary validation
K562 Bethyl_A301-274A_4_CPSF3L.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CPSF3L specific antibody, A301-274A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CPSF3L-specific antibody, A301-274A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the CPSF3L specific antibody, A301-274A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CPSF3L-specific antibody, A301-274A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

Cell linePrimary validationSecondary validation
HepG2 CPSF3L-HEPG2-CRISPR-A301-274A.png<br>Caption: Western blot following CRISPR against INTS11 in HepG2 whole cell lysate using INTS11 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against INTS11. INTS11 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against INTS11 in HepG2 whole cell lysate using INTS11 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against INTS11. INTS11 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHcLV34-13

Status: Released

Lab: Graveley Lab