SUB1 antibody: A301-161A


Antibody Info
Product_IDA301-161A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB835QRD
Target Info
GeneNameSUB1
Phosphate
Target_nameSUB1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-161A_1_SUB1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SUB1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SUB1 antibody (lanes under 'SUB1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SUB1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SUB1 antibody (lanes under 'SUB1').

Method: immunoprecipitation

Releated Sample: eCLIP:632

Status: Released

Lab: Yeo Lab

SUB1_Secondary_Western.png<br>Caption: Western blot following shRNA against SUB1 in K562 and HepG2 whole cell lysate using SUB1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SUB1. Lanes 5-8 follow the same pattern, but in HepG2. SUB1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against SUB1 in K562 and HepG2 whole cell lysate using SUB1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SUB1. Lanes 5-8 follow the same pattern, but in HepG2. SUB1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV13-41

Status: Released

Lab: Graveley Lab

K562 SUB1_Secondary_Western.png<br>Caption: Western blot following shRNA against SUB1 in K562 and HepG2 whole cell lysate using SUB1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SUB1. Lanes 5-8 follow the same pattern, but in HepG2. SUB1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against SUB1 in K562 and HepG2 whole cell lysate using SUB1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SUB1. Lanes 5-8 follow the same pattern, but in HepG2. SUB1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV13-41

Status: Released

Lab: Graveley Lab