Cell line | Primary validation | Secondary validation |
---|---|---|
HepG2 | ![]() Caption: Western blot following shRNA against SAFB2 in HepG2 whole cell lysate using SAFB2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SAFB2. SAFB2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGHLV20-53 Status: Released Lab: Graveley Lab | |
K562 | ![]() Caption: IP-Western Blot analysis of K562 whole cell lysate using SAFB2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SAFB2 antibody (lanes under 'SAFB2'). Method: immunoprecipitation Releated Sample: eCLIP:267 Status: Released Lab: Yeo Lab | ![]() Caption: Western blot following shRNA against SAFB2 in K562 whole cell lysate using SAFB2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SAFB2. SAFB2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKLV24-51 Status: Released Lab: Graveley Lab |