| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AATF specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AATF antibody (lanes under 'AATF'). Method: immunoprecipitation Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against AATF in K562 and HepG2 whole cell lysate using AATF specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against AATF. Lanes 5-7 follow the same pattern, but in HepG2. AATF protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV13-13 Status: Released Lab: Graveley Lab |
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| K562 |
Caption: IP-Western Blot analysis of K562 whole cell lysate using AATF specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AATF antibody (lanes under 'AATF'). Method: immunoprecipitation Releated Sample: eCLIP:511 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against AATF in K562 and HepG2 whole cell lysate using AATF specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against AATF. Lanes 5-7 follow the same pattern, but in HepG2. AATF protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV13-13 Status: Released Lab: Graveley Lab |
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